Heparan sulfate is a selective attachment factor for the avian coronavirus infectious bronchitis virus Beaudette.
Identifieur interne : 003695 ( Main/Exploration ); précédent : 003694; suivant : 003696Heparan sulfate is a selective attachment factor for the avian coronavirus infectious bronchitis virus Beaudette.
Auteurs : Ikenna G. Madu [États-Unis] ; Victor C. Chu ; Hwajin Lee ; Andrew D. Regan ; Beverley E. Bauman ; Gary R. WhittakerSource :
- Avian diseases [ 0005-2086 ] ; 2007.
Descripteurs français
- KwdFr :
- Animaux, Biologie informatique, Bovins, Cricetinae, Données de séquences moléculaires, Embryon de poulet, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (métabolisme), Héparitine sulfate (métabolisme), Liaison aux protéines, Lignée cellulaire, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (métabolisme), Récepteurs de surface cellulaire (), Récepteurs de surface cellulaire (métabolisme), Séquence d'acides aminés, Virus de la bronchite infectieuse (), Virus de la bronchite infectieuse (physiologie).
- MESH :
- métabolisme : Glycoprotéines membranaires, Héparitine sulfate, Protéines de l'enveloppe virale, Récepteurs de surface cellulaire.
- physiologie : Virus de la bronchite infectieuse.
- Animaux, Biologie informatique, Bovins, Cricetinae, Données de séquences moléculaires, Embryon de poulet, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Liaison aux protéines, Lignée cellulaire, Protéines de l'enveloppe virale, Récepteurs de surface cellulaire, Séquence d'acides aminés, Virus de la bronchite infectieuse.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Cattle, Cell Line, Chick Embryo, Computational Biology, Cricetinae, Heparitin Sulfate (metabolism), Infectious bronchitis virus (classification), Infectious bronchitis virus (physiology), Membrane Glycoproteins (chemistry), Membrane Glycoproteins (metabolism), Molecular Sequence Data, Protein Binding, Receptors, Cell Surface (chemistry), Receptors, Cell Surface (metabolism), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Receptors, Cell Surface, Viral Envelope Proteins.
- chemical , metabolism : Heparitin Sulfate, Membrane Glycoproteins, Receptors, Cell Surface, Viral Envelope Proteins.
- classification : Infectious bronchitis virus.
- physiology : Infectious bronchitis virus.
- Amino Acid Sequence, Animals, Cattle, Cell Line, Chick Embryo, Computational Biology, Cricetinae, Molecular Sequence Data, Protein Binding, Spike Glycoprotein, Coronavirus.
Abstract
The avian coronavirus infectious bronchitis virus (IBV) strain Beaudette is an embryo-adapted virus that has extended species tropism in cell culture. In order to understand the acquired tropism of the Beaudette strain, we compared the S protein sequences of several IBV strains. The Beaudette strain was found to contain a putative heparan sulfate (HS)-binding site, indicating that the Beaudette virus may use HS as a selective receptor. To ascertain the requirements of cell-surface HS for Beaudette infectivity, we assayed for infectivity in the presence of soluble heparin as a competitor and determined infectivity in mutant cell lines with no HS or glycosaminoglycan expression. Our results indicate that HS plays a role as an attachment factor for IBV, working in concert with other factors like sialic acid to mediate virus binding to cells, and may explain in part the extended tropism of IBV Beaudette.
DOI: 10.1637/0005-2086(2007)051[0045:HSIASA]2.0.CO;2
PubMed: 17461266
Affiliations:
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Madu</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853</wicri:regionArea><placeName><region type="state">État de New York</region><settlement type="city">Ithaca (New York)</settlement></placeName><orgName type="university">Université Cornell</orgName></affiliation></author><author><name sortKey="Chu, Victor C" sort="Chu, Victor C" uniqKey="Chu V" first="Victor C" last="Chu">Victor C. Chu</name></author><author><name sortKey="Lee, Hwajin" sort="Lee, Hwajin" uniqKey="Lee H" first="Hwajin" last="Lee">Hwajin Lee</name></author><author><name sortKey="Regan, Andrew D" sort="Regan, Andrew D" uniqKey="Regan A" first="Andrew D" last="Regan">Andrew D. Regan</name></author><author><name sortKey="Bauman, Beverley E" sort="Bauman, Beverley E" uniqKey="Bauman B" first="Beverley E" last="Bauman">Beverley E. Bauman</name></author><author><name sortKey="Whittaker, Gary R" sort="Whittaker, Gary R" uniqKey="Whittaker G" first="Gary R" last="Whittaker">Gary R. Whittaker</name></author></analytic><series><title level="j">Avian diseases</title><idno type="ISSN">0005-2086</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cattle</term><term>Cell Line</term><term>Chick Embryo</term><term>Computational Biology</term><term>Cricetinae</term><term>Heparitin Sulfate (metabolism)</term><term>Infectious bronchitis virus (classification)</term><term>Infectious bronchitis virus (physiology)</term><term>Membrane Glycoproteins (chemistry)</term><term>Membrane Glycoproteins (metabolism)</term><term>Molecular Sequence Data</term><term>Protein Binding</term><term>Receptors, Cell Surface (chemistry)</term><term>Receptors, Cell Surface (metabolism)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Biologie informatique</term><term>Bovins</term><term>Cricetinae</term><term>Données de séquences moléculaires</term><term>Embryon de poulet</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires ()</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Héparitine sulfate (métabolisme)</term><term>Liaison aux protéines</term><term>Lignée cellulaire</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Récepteurs de surface cellulaire ()</term><term>Récepteurs de surface cellulaire (métabolisme)</term><term>Séquence d'acides aminés</term><term>Virus de la bronchite infectieuse ()</term><term>Virus de la bronchite infectieuse (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term><term>Receptors, Cell Surface</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Heparitin Sulfate</term><term>Membrane Glycoproteins</term><term>Receptors, Cell Surface</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Infectious bronchitis virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Héparitine sulfate</term><term>Protéines de l'enveloppe virale</term><term>Récepteurs de surface cellulaire</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus de la bronchite infectieuse</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Infectious bronchitis virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cattle</term><term>Cell Line</term><term>Chick Embryo</term><term>Computational Biology</term><term>Cricetinae</term><term>Molecular Sequence Data</term><term>Protein Binding</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Biologie informatique</term><term>Bovins</term><term>Cricetinae</term><term>Données de séquences moléculaires</term><term>Embryon de poulet</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Liaison aux protéines</term><term>Lignée cellulaire</term><term>Protéines de l'enveloppe virale</term><term>Récepteurs de surface cellulaire</term><term>Séquence d'acides aminés</term><term>Virus de la bronchite infectieuse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The avian coronavirus infectious bronchitis virus (IBV) strain Beaudette is an embryo-adapted virus that has extended species tropism in cell culture. In order to understand the acquired tropism of the Beaudette strain, we compared the S protein sequences of several IBV strains. The Beaudette strain was found to contain a putative heparan sulfate (HS)-binding site, indicating that the Beaudette virus may use HS as a selective receptor. To ascertain the requirements of cell-surface HS for Beaudette infectivity, we assayed for infectivity in the presence of soluble heparin as a competitor and determined infectivity in mutant cell lines with no HS or glycosaminoglycan expression. Our results indicate that HS plays a role as an attachment factor for IBV, working in concert with other factors like sialic acid to mediate virus binding to cells, and may explain in part the extended tropism of IBV Beaudette.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region><settlement><li>Ithaca (New York)</li></settlement><orgName><li>Université Cornell</li></orgName></list><tree><noCountry><name sortKey="Bauman, Beverley E" sort="Bauman, Beverley E" uniqKey="Bauman B" first="Beverley E" last="Bauman">Beverley E. Bauman</name><name sortKey="Chu, Victor C" sort="Chu, Victor C" uniqKey="Chu V" first="Victor C" last="Chu">Victor C. Chu</name><name sortKey="Lee, Hwajin" sort="Lee, Hwajin" uniqKey="Lee H" first="Hwajin" last="Lee">Hwajin Lee</name><name sortKey="Regan, Andrew D" sort="Regan, Andrew D" uniqKey="Regan A" first="Andrew D" last="Regan">Andrew D. Regan</name><name sortKey="Whittaker, Gary R" sort="Whittaker, Gary R" uniqKey="Whittaker G" first="Gary R" last="Whittaker">Gary R. Whittaker</name></noCountry><country name="États-Unis"><region name="État de New York"><name sortKey="Madu, Ikenna G" sort="Madu, Ikenna G" uniqKey="Madu I" first="Ikenna G" last="Madu">Ikenna G. Madu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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